The UK-wide ITV debate will take place next Thursday, two weeks before the poll on EU membership.
The two-hour programme is scheduled to be similar to the general election leaders’ debates, with the full line-up still to be finalised.
Some reports suggest the Brexit side could include Boris Johnson.
Ms Sturgeon said: “I want to make the positive, progressive case from a Scottish perspective for remaining in Europe.”
She added: ”Scotland and the UK benefit enormously from our place in Europe – from important social protections such as employment rights and parental leave to membership of the world’s largest single market and I will continue to make the positive case for a vote to remain on the ITV debate and for the rest of the campaign.”
The Scottish Vote Leave campaign says Scotland would get “major” new powers and a bigger budget if the UK votes to leave the European Union.
The campaign claims the UK pays £350m a week to the EU for the “privilege of maintaining its membership” and that Scotland’s share was £1.5bn a year.
